Series A AI & Machine Learning Venture Capital Firms

Early-stage firm investing in digital health and data/security infrastructure, including healthcare IT and enterprise SaaS.

• Healthcare
• Other
• Security

• Seed, Series A
• $750K – $6.0M
• Boston, MA
• 97 portfolio companies

Operator-led enterprise fund investing in technical B2B software, including security and infrastructure products. Helpful for founders needing enterprise GTM and scaling support.

• Enterprise
• Security
• Developer Tools

• Seed, Series A
• $500K – $3.0M
• San Francisco, CA
• 13 portfolio companies

511 Ventures focuses on AI-enabled enterprise infrastructure and tools aimed at safety, stability, and optimization within high-consequence B2B verticals.

• Other
• Deep Tech
• SaaS

• Seed, Series A
• $250K – $2.5M
• Boston, MA
• 1 portfolio company

ACME Capital supports early-stage companies modernizing infrastructure, enterprise technologies, and industrial workflows.

• SaaS
• AI / ML
• Other

• Seed, Series A
• $1.0M – $3.0M
• San Francisco, CA
• 8 portfolio companies

Backing AI-driven technologies across industries, with a key emphasis on enterprise and B2B intelligence solutions.

• AI / ML
• SaaS

• seed+, Series A
• $1.0M – $5.0M
• San Francisco, CA
• 37 portfolio companies

Invests in startups using AI to spearhead innovations in cybersecurity, enhancing threat detection and response capabilities.

• Security
• Other
• AI / ML

• Seed, Series A
• $500K – $3.0M
• Chicago, IL
• 16 portfolio companies

Dedicated to advancing AI-centric fintech startups that improve financial workflows, debt solutions, and operational efficiencies.

• AI / ML
• Fintech
• Other

• Seed, Series A
• $1.0M – $7.0M
• Atlanta, GA
• 2 portfolio companies

AI First Capital specializes in early-stage companies using artificial intelligence to solve industry-specific problems, focusing on verticalized AI applications.

• AI / ML
• SaaS
• Other

• Pre-Seed, Seed, Series A
• $500K – $3.0M
• Palo Alto, CA
• 8 portfolio companies

Specializes in AI-driven industrial inspection technologies that enhance operational safety and efficiency.

• Other
• AI / ML

• Seed, Series A
• $300K – $3.5M
• Boston, MA
• 1 portfolio company

Invests in AI-native platforms with infrastructural applications for enterprise software systems and integrations.

• AI / ML
• SaaS

• Seed, Series A
• $500K – $3.0M
• New York, NY
• 1 portfolio company

Focuses exclusively on companies propelling AI technologies for use cases in marketing and enterprise visibility solutions.

• SaaS
• Other
• AI / ML

• Seed, Series A
• $1.0M – $3.0M
• Toronto, Canada
• 1 portfolio company

Invests in early-stage companies using AI to transform manufacturing processes and capabilities.

• AI / ML
• Other
• SaaS

• Pre-Seed, Seed, Series A
• $300K – $3.0M
• Tokyo, Japan
• 2 portfolio companies

Specialized in AI/ML startups with productivity-focused applications, especially those targeting operational efficiency in commerce and logistics sectors.

• AI / ML
• Other

• Seed, Series A
• $500K – $3.0M
• New York, NY
• 3 portfolio companies

Specializes in climate-resilient tech and sustainable infrastructure for industrial and global applications.

• Other
• Developer Tools
• AI / ML

• Seed, Series A
• $1.0M – $5.0M
• Singapore
• 2 portfolio companies

Focused exclusively on transformative healthcare technology solutions that address hospital challenges and improve care delivery via AI and SaaS models.

• Other
• Healthcare
• SaaS

• Seed+, Series A
• $3.0M – $10.0M
• Boston, MA
• 2 portfolio companies

Fostering early-stage innovations in agricultural technology through strategic funding of startups poised to optimize food supply chains.

• Food / AgTech
• Fintech
• AI / ML

• Seed, Series A
• $500K – $3.0M
• London, UK
• 1 portfolio company

Investing in groundbreaking startups in AI-enabled platforms, consumer social products, and creator ecosystems globally.

• AI / ML
• Other
• Marketplace

• Pre-Seed, Seed, Series A
• $500K – $5.0M
• Sydney, Australia
• 49 portfolio companies

Stealth fund backing AI-first SaaS companies that solve tangible everyday productivity challenges.

• Other
• SaaS
• AI / ML

• Seed, Series A
• $500K – $5.0M
• San Francisco, CA
• 31 portfolio companies

Focused on Enterprise SaaS, infrastructure, and tools enabling AI-driven workflows for regulated industries.

• Enterprise
• Other
• SaaS

• Seed, Series A
• $500K – $3.0M
• San Francisco, CA
• 47 portfolio companies

Focuses on ventures rethinking the future of commerce and retail sustainability, including furniture, home goods, and fashion.

• Other
• Climate / Energy
• AI / ML

• Seed, Series A
• $500K – $3.0M
• Chicago, IL
• 16 portfolio companies

AlignThesis backs startups proving measurable productivity gains in enterprise environments, including vertical SaaS targeting HR and people operations.

• Other
• SaaS
• Enterprise

• Seed, Series A
• $700K – $5.0M
• Chicago, IL
• 2 portfolio companies

Invests at the intersection of enterprise SaaS and AI/ML innovation, targeting scalable B2B solutions.

• SaaS
• AI / ML

• seed+, Series A
• $1.5M – $3.0M
• New York, NY
• 12 portfolio companies

Focuses on early-stage investments in AI-backed solutions transforming B2B marketing, sales, and customer engagement strategies.

• Other
• AI / ML
• SaaS

• Seed, Series A
• $300K – $1.5M
• Chicago, IL
• 2 portfolio companies

Focuses on B2B SaaS startups leveraging data and AI-driven automation.

• SaaS
• Marketplace
• AI / ML

• Seed, Series A
• $300K – $2.0M
• New York, NY
• 2 portfolio companies